Methods of producing nucleic acid molecules comprising stem loop oligonucleotides
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United States of America Patent
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Sep 28, 2010
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Nov 13, 2008
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Aug 2, 2005
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Abstract
The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
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